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Effects of eluent composition ion pair reagent and temperature on the separation of histones by HPLC
Summary
Type:
HPLC
Application #:
108540
Detection:
UV, 210nm, 0.1 AUFS
Conditions:
a) eluent A: water containing 0.1% trifluoroacetic acid (TFA), eluent B: 70% acetonitrile with 0.1% TFA,
b) eluent A: water containing 10% EGME and 0.1% TFA, eluent B: EGME - 70% acetonitrile (10:90) with 0.1% TFA, linear gradient for both: 40 - 52% B in 25min
flow rate 0.5 ml/min
Authors:
Lindner H
Source:
Chromatographia 30 (1990) 518 - 522
Details
Legend: Fig. 2. Effect of ethylene glycol monomethyl ether (EGME) on separation of H1 histones and HMG proteins from Friend erythroleukaemic cells (lines F4N), 1. histone H1°, 2. histone H1b, 3. HMG protein
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